Characterization of Parallel β-Sheets at Interfaces by Chiral Sum Frequency Generation Spectroscopy.

نویسندگان

  • Li Fu
  • Zhuguang Wang
  • Brian T Psciuk
  • Dequan Xiao
  • Victor S Batista
  • Elsa C Y Yan
چکیده

Characterization of protein secondary structures at interfaces is still challenging due to the limitations of surface-selective optical techniques. Here, we address the challenge of characterizing parallel β-sheets by combining chiral sum frequency generation (SFG) spectroscopy and computational modeling. We focus on human islet amyloid polypeptide aggregates and a de novo designed short polypeptide at lipid/water and air/glass interfaces. We find that parallel β-sheets adopt distinct orientations at various interfaces and exhibit characteristic chiroptical responses in the amide I and N-H stretch regions. Theoretical analysis indicates that the characteristic chiroptical responses provide valuable information on the symmetry, orientation, and vibrational couplings of parallel β-sheet at interfaces.

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عنوان ژورنال:
  • The journal of physical chemistry letters

دوره 6 8  شماره 

صفحات  -

تاریخ انتشار 2015